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CompuGene is an interdisciplinary consortium of natural science and engineering with the aim to develop computer-aided designs of complex, synthetic genetic circuits. In this framework it is the task of the kabisch-lab to develop methods for the rapid prototyping of these circuits. For this we examined a variety of DNA assembly methods in respect to their automation potential. After identifying the ligase cycling reaction (LCR) as most suited we have begun to evaluate the limits of the method, started building models, as well as creating computer-aided design tools enabling us model optimal assembly designs. Furthermore we down-scaled the DNA assembly reactions to 1 µl using a nanoliter dispenser.
We research and optimize oleaginous microorganisms such as the yeast Yarrowia lipolytica in respect to their oil body formation capabilities and methods to transform these into a renewable gasoline replacements. Our second host, the Gram-positive bacterium Bacillus subtilis, is being optimized with the goal to produce hydrocarbons of varying length.
Deciphering the physiological function of fundamental cellular processes has until recently been hindered by the lack of molecular tools to manipulate essential genes in vivo. Recent breakthroughs in the technology for genome engineering, such as CRISPR-Cas9, have allowed the characterization of these under-studied processes. In the frame of this project we propose to use these new tools for the creation of a cell that will lose central mechanisms of the bacterial life cycle.
Will not be disclosed.